Clinical Histopathological and Immunohistochemistry of papillomavirus cutaneous lesions in animals in Al Muthanna veterinary hospital/ Iraq
Clinical Histopathological and Immunohistochemistry of papillomavirus cutaneous lesions in animals in Al Muthanna veterinary hospital/ Iraq
Blog Article
Papilloma viruses (PVs) are non-enveloped, small, double stranded DNA viruses with circular genomes that replicate intranuclearly in infected cells.Papilloma viruses have high affinity for stratified squamous epithelia of warm blooded animals including cattle and occurs in all animal species throughout the world, and are highly species-specific.However, the only reported cases of cross-species infection had occurred in horses and other equids.Bovine cutaneous papillomatosis is a common skin disease in Iraq.The regulation of cells undergoing apoptosis is controlled by the bcl-2 proteins family; it includes both pro- (i.
e.Bax, Bak) and antiapoptotic proteins (i.e.bcl-2 itself ) triggering or blocking, respectively, the release from mythocondria of proteins activating the apoptotic molecular cascade.P53 is a tumor suppressor protein that mediates cell cycle arrest, DNA integrity and repairing, apoptosis and senescence in response to specific cellular stresses (e.
g.various types of DNA damage, hypoxia, oncogene deregulation, oxidative damage).P53 has a significant role in cell cycle control, therefore the loss of its suppressive function has been reported for different types of human neoplasia and animal tumours, such as BPV induced tumours.The aim of the present study was to describe the clinical, histopathological and riley reid schoolgirl immunohistochemical aspects of naturally occurring cutaneous papillomatosis in bovine, ovine and caprine.A total of 3 cattle, 3 goats and 4 sheep were presented to Al Muthanna Veterinary Hospital with multiple cutaneous lesions that were diagnosed as cutaneous papilloma by clinical examination.
The exophytic multiple, cauliflower- like growths (warts ) of varying sizes (0.5-11 cm) were found in different areas of the body in bovine.Moreover, the lesions were found ultimate warrior hat in the lips, genital, shoulder and chest in sheep and goat.Histopathology revealed various degrees of koilocytosis, ortho and parakeratotic, hyperkeratosis, hypergranulosis in granular layer and acanthosis, suggesting a cytopathic action of BPV in all neoplasms.Immunohistochemical (IHC) examination revealed some nuclei in the granular and basal layers of the epidermis that showed intense positivity for papilloma virus antigen.
The tumour samples were also stained positively for bcl-2 throughout basal and parabasal layers, with most of cells showing strong cytoplasmic immunoreactivity.All tumor samples were found to be positive for p53 expression, showing a strong cytoplasmic and perinuclear staining of p53 protein mainly in the basal and parabasal layers.In conclusion, papilloma virus occurred in bovine, ovine and caprine in Al muthanna province that revealed different anatomical clinical presentation and similar histopathological features in all animals with minor variation.Moreover, tumor samples revealed positive results for papilloma virus antigen, bcl-2 and p53 markers that can be considered as very useful markers in the diagnosis of bovine cutaneous papilloma.